Composite

Part:BBa_K4757070:Design

Designed by: Marik Müller   Group: iGEM23_Heidelberg   (2023-12-14)


Constructs for testing of different sRNAs targeting mKate2 and/or the RBS BBa_J61101


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 388
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 925
    Illegal SapI site found at 1255
    Illegal SapI.rc site found at 1229


Design Notes

Source

Sequences were obtained through synthesis

References