Composite
Part:BBa_K4757070:Design
Designed by: Marik Müller Group: iGEM23_Heidelberg (2023-12-14)
Constructs for testing of different sRNAs targeting mKate2 and/or the RBS BBa_J61101
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1110
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 388
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 925
Illegal SapI site found at 1255
Illegal SapI.rc site found at 1229
Design Notes
Source
Sequences were obtained through synthesis